Journal: Glycobiology

Article Title: Elucidating the unusual reaction kinetics of D-glucuronyl C5-epimerase

doi: 10.1093/glycob/cwaa035

Figure Lengend Snippet: C5-epimerase catalyzes the conversion of GlcA to IdoA. This reaction is reversible and is skewed towards the formation of the more thermodynamically favored GlcA. (A) Forward reaction using NSH and (B) pseudo-reverse reaction using CDNSH. The protons deuterium exchanged at the C5 positions of the GlcA and the IdoA residues are shown in blue. (C) NMR-based assay that is used to detect directly the presence of GlcA and IdoA within the reaction based on differences in chemical shifts of the anomeric proton of GlcA and IdoA. The peak area as a function of the internal standard (DSS) provides quantitative amounts (in mM) of GlcA and IdoA within the reaction. This figure is available in black and white in print and in color at Glycobiology online.

Article Snippet: For all NMR experiments, 10 μM DSS (Cambridge Isotope Laboratories, Andover, MA) was used as an internal standard for quantification of GlcA and IdoA concentrations.

Techniques:

Journal: iScience

Article Title: Sirtuin 1/sirtuin 3 are robust lysine delactylases and sirtuin 1-mediated delactylation regulates glycolysis

doi: 10.1016/j.isci.2024.110911

Figure Lengend Snippet:

Article Snippet: DSS , Sangon Biotech , C100015-0100.

Techniques: Virus, Recombinant, Transfection, Marker, Kinase Assay, CCK-8 Assay, Software, Microscopy

Journal: Frontiers in Physiology

Article Title: Slight Disruption in Intestinal Environment by Dextran Sodium Sulfate Reduces Egg Yolk Size Through Disfunction of Ovarian Follicle Growth

doi: 10.3389/fphys.2020.607369

Figure Lengend Snippet: Histology and Occludin mRNA expression in the intestinal mucosa with or without dextran sodium sulfate (DSS). Micrographs demonstrate the HE staining of the cecum [Cec: (A) and (B) ] and rectum [Rec: (C) and (D) ] in hens orally administered DSS (DSS group) or water (CON group). E = mucosal epithelium, L = lumen, LP = lamina propria. Scale bars = 50 μm. (E) Ratio of villus height/ crypt depth of jejunum (Jej), ileum (Ile), Cec, and Rec. Open bars represent the control group, and orange filled bars represent the DSS group. Values are the ratio of villus height/crypt depth (CON and DSS, n = 8 and 7). (F) Effects of the oral administration of DSS on the mRNA expression of Occludin in the intestinal mucosa. Values are the fold change in target gene expression when compared to a standard sample from the CON group of each segment (CON and DSS, n = 8 and 7). Target gene expression was normalized to the house-keeping gene RPS17 . Asterisks (*, **) indicate significant differences between the CON and DSS groups ( P < 0.05 and P < 0.01, respectively).

Article Snippet: Birds were administered a single oral dose of sterilized water with or without 0.225 g DSS/kg body weight (molecular weight: 5,000–6,000, Nacalai Tesque, Inc., Kyoto, Japan) by cannula for 28 days (DSS and CON groups, respectively).

Techniques: Expressing, Staining, Control, Targeted Gene Expression

Journal: Frontiers in Physiology

Article Title: Slight Disruption in Intestinal Environment by Dextran Sodium Sulfate Reduces Egg Yolk Size Through Disfunction of Ovarian Follicle Growth

doi: 10.3389/fphys.2020.607369

Figure Lengend Snippet: The effects of dextran sodium sulphate (DSS) on the mRNA expression of pro- [IL-1β and IL-6: (A) and (B) ] and anti- [TGFβ-2 and 4: (C) and (D) ] inflammatory cytokines in the mucosal tissues of the jejunum (Jej), ileum (Ile), cecum (Cec), and rectum (Rec). The effects of dextran sodium sulfate (DSS) on the mRNA expression of pro- and anti-inflammatory cytokines in the mucosal tissues of the jejunum (Jej), ileum (Ile), cecum (Cec), and rectum (Rec). Open bars represent the CON group, and orange filled bars represent the DSS group. Values are the fold change in the target gene expression when compared to a standard sample from the CON group of each segment (CON and DSS, n = 8 and 7). The target gene expression was normalized to the house-keeping gene RPS17 . Asterisks (*) indicate significant differences between the water (CON) and DSS-treated groups ( P < 0.05).

Article Snippet: Birds were administered a single oral dose of sterilized water with or without 0.225 g DSS/kg body weight (molecular weight: 5,000–6,000, Nacalai Tesque, Inc., Kyoto, Japan) by cannula for 28 days (DSS and CON groups, respectively).

Techniques: Expressing, Targeted Gene Expression

Journal: Frontiers in Physiology

Article Title: Slight Disruption in Intestinal Environment by Dextran Sodium Sulfate Reduces Egg Yolk Size Through Disfunction of Ovarian Follicle Growth

doi: 10.3389/fphys.2020.607369

Figure Lengend Snippet: Microbiome profiles in the cecum contents of laying hens administered dextran sodium sulfate (DSS group) or water (CON group). (A) A taxonomic diversity bar plot showing relative abundance of taxa identified to the family level ( n = 4). (B) Alpha-diversity boxplot of observed OTUs. Boxplots show the quartiles, median, and extremities of the value. Asterisk (*) indicate significant differences between the water (CON) and DSS-treated groups ( P < 0.05). (C) 3D PCoA plot based on an unweighted UniFrac distance matrix. White circles represent the CON group, and orange circles represent the DSS group.

Article Snippet: Birds were administered a single oral dose of sterilized water with or without 0.225 g DSS/kg body weight (molecular weight: 5,000–6,000, Nacalai Tesque, Inc., Kyoto, Japan) by cannula for 28 days (DSS and CON groups, respectively).

Techniques:

Journal: Frontiers in Physiology

Article Title: Slight Disruption in Intestinal Environment by Dextran Sodium Sulfate Reduces Egg Yolk Size Through Disfunction of Ovarian Follicle Growth

doi: 10.3389/fphys.2020.607369

Figure Lengend Snippet: Changes in the egg (A) , egg-yolk (B) , egg-shell (C) , and egg-white (D) weight in hens administered dextran sodium sulphate (DSS group) or water (CON group). Open circle plots represent the CON group, and the orange rhombus plots represent the DSS group. Values are the mean ± SEM (CON and DSS, n = 8 and 7). Asterisks (**) indicate significant differences between the CON and DSS groups at the same time point ( P < 0.01). Lower case letters ( a,b ) indicate significant differences between the time points ( P < 0.05).

Article Snippet: Birds were administered a single oral dose of sterilized water with or without 0.225 g DSS/kg body weight (molecular weight: 5,000–6,000, Nacalai Tesque, Inc., Kyoto, Japan) by cannula for 28 days (DSS and CON groups, respectively).

Techniques:

Journal: Frontiers in Physiology

Article Title: Slight Disruption in Intestinal Environment by Dextran Sodium Sulfate Reduces Egg Yolk Size Through Disfunction of Ovarian Follicle Growth

doi: 10.3389/fphys.2020.607369

Figure Lengend Snippet: The effects of the oral administration of dextran sodium sulfate (DSS) on hepatic histology and function in hens. Micrographs of the liver tissue of hens treated with water [ (A) ; CON group] and DSS [ (B) ; DSS group]. HE staining. Scale bars = 50 μm. (C) Effects of the oral administration of DSS on the mRNA expression of egg yolk precursor related genes. Open bars represent the CON group, and orange filled bars represent the DSS group. Values demonstrate the fold change in the target gene expression when compared to a standard sample from the CON group of each segment (CON and DSS, n = 8 and 7). Target gene expression was normalized to the house-keeping gene RPS17 . Asterisk (*) indicate significant difference between the control and DSS groups ( P < 0.05).

Article Snippet: Birds were administered a single oral dose of sterilized water with or without 0.225 g DSS/kg body weight (molecular weight: 5,000–6,000, Nacalai Tesque, Inc., Kyoto, Japan) by cannula for 28 days (DSS and CON groups, respectively).

Techniques: Staining, Expressing, Targeted Gene Expression, Control

Journal: Frontiers in Physiology

Article Title: Slight Disruption in Intestinal Environment by Dextran Sodium Sulfate Reduces Egg Yolk Size Through Disfunction of Ovarian Follicle Growth

doi: 10.3389/fphys.2020.607369

Figure Lengend Snippet: The plasma parameters of triglyceride (TG) and total cholesterol (T-CHO). Changes in plasma TG (A) and T-CHO (B) during the experimental period in hens administered dextran sodium sulfate (DSS group) or water (CON group). Open circle plots represent the control group, and the orange rhombus plots represent the DSS group. Values are the mean ± SEM (CON and DSS, n = 8 and 7). (C) The concentration of TG and cholesterol (CHO) in VLDLy (particle size with diameter of 25–44 nm) separated by high performance liquid chromatography (HPLC). Open circle plots represent the CON group, and the orange rhombus plots represent the DSS group. Asterisks (*, **) indicate significant differences between the control and DSS groups at the same time point ( P < 0.05 and P < 0.01, respectively). Lower case letters ( a−c ) indicate significant differences between the time points ( P < 0.05).

Article Snippet: Birds were administered a single oral dose of sterilized water with or without 0.225 g DSS/kg body weight (molecular weight: 5,000–6,000, Nacalai Tesque, Inc., Kyoto, Japan) by cannula for 28 days (DSS and CON groups, respectively).

Techniques: Clinical Proteomics, Control, Concentration Assay, High Performance Liquid Chromatography

Journal: Frontiers in Physiology

Article Title: Slight Disruption in Intestinal Environment by Dextran Sodium Sulfate Reduces Egg Yolk Size Through Disfunction of Ovarian Follicle Growth

doi: 10.3389/fphys.2020.607369

Figure Lengend Snippet: Effects of dextran sodium sulfate (DSS) on the mRNA expression of the tight-junction [ (A) and (B) ] and lipoprotein receptors [ (C) and (D) ] in the granulosa cells of F1 and F5 follicles. Open bars represent the CON group, and orange filled bars represent the DSS group. Values are the fold change in target gene expression when compared to a standard sample from the CON group of each segments (CON and DSS, n = 8 and 7). Target gene expression was normalized to the house-keeping gene RPS17 . Asterisks (*, **) indicate significant differences between the water (control) and DSS-treated groups ( P < 0.05 and P < 0.01, respectively).

Article Snippet: Birds were administered a single oral dose of sterilized water with or without 0.225 g DSS/kg body weight (molecular weight: 5,000–6,000, Nacalai Tesque, Inc., Kyoto, Japan) by cannula for 28 days (DSS and CON groups, respectively).

Techniques: Expressing, Targeted Gene Expression, Control

Journal: Frontiers in Physiology

Article Title: Slight Disruption in Intestinal Environment by Dextran Sodium Sulfate Reduces Egg Yolk Size Through Disfunction of Ovarian Follicle Growth

doi: 10.3389/fphys.2020.607369

Figure Lengend Snippet: Effects of dextran sodium sulfate (DSS) on the mRNA expression of gonadotropin and receptor (A) and interleukin-1β (B) in the anterior pituitary gland. Values are the fold change in target gene expression when compared to a standard sample from the CON group of each segments (CON and DSS, n = 8 and 7). Target gene expression was normalized to the house-keeping gene RPS17 . Asterisk (*) indicate significant differences between the water (control) and DSS-treated groups ( P < 0.05).

Article Snippet: Birds were administered a single oral dose of sterilized water with or without 0.225 g DSS/kg body weight (molecular weight: 5,000–6,000, Nacalai Tesque, Inc., Kyoto, Japan) by cannula for 28 days (DSS and CON groups, respectively).

Techniques: Expressing, Targeted Gene Expression, Control

Journal: Frontiers in Physiology

Article Title: Slight Disruption in Intestinal Environment by Dextran Sodium Sulfate Reduces Egg Yolk Size Through Disfunction of Ovarian Follicle Growth

doi: 10.3389/fphys.2020.607369

Figure Lengend Snippet: Changes in plasma corticosterone (A) , estradiol-17β (B) , and lipopolysaccharide binding protein (LBP) (C) during the experimental period in hens administered dextran sodium sulphate (DSS group) or water (CON group). Open circle plots represent the control group, and the orange rhombus plots represent the DSS group. Values are the mean ± SEM (CON and DSS, n = 8 and 7). Lower case letters ( a,b ) indicate significant differences between the time points ( P < 0.05).

Article Snippet: Birds were administered a single oral dose of sterilized water with or without 0.225 g DSS/kg body weight (molecular weight: 5,000–6,000, Nacalai Tesque, Inc., Kyoto, Japan) by cannula for 28 days (DSS and CON groups, respectively).

Techniques: Clinical Proteomics, Binding Assay, Control